human umbilical cord mesenchymal stem cells (uc-mscs)  (Procell Inc)

Journal: Materials Today Bio

Article Title: ROS-scavenging ultrasonicated graphene oxide/alginate microgels for mesenchymal stem cell delivery and hindlimb ischemia treatment

doi: 10.1016/j.mtbio.2024.101289

Figure Lengend Snippet: Schematic illustration depicting sonicated graphene oxide/alginate (sGO/alginate) microgels that embed mesenchymal stem cells (MSCs) for the repair of ischemic hindlimbs. ROS-scavenging sGO/alginate microgels can protect encapsulated MSCs from the high oxidative stress in ischemic tissues and facilitate tissue regeneration by enabling the MSCs to produce angiogenic factors at injury sites.

Article Snippet: Human umbilical cord-derived MSCs and human umbilical vein endothelial cells (HUVEC) were obtained from PromoCell (Heidelberg, Germany).

Techniques: Sonication

Journal: Materials Today Bio

Article Title: ROS-scavenging ultrasonicated graphene oxide/alginate microgels for mesenchymal stem cell delivery and hindlimb ischemia treatment

doi: 10.1016/j.mtbio.2024.101289

Figure Lengend Snippet: Viability of the MSCs encapsulated in various microgels under normal and oxidative stress conditions. Sample groups include MSCs encapsulated in alginate microgels (MSC/alginate), MSCs encapsulated GO/alginate microgels (MSC/GO/alginate), and MSCs encapsulated sGO/alginate microgels (MSC/sGO/alginate). (a) Live/dead staining images and (b) cell viability 24 h after culture in the absence or presence of ROS substances (H 2 O 2 , menadione, or TBHP) (n = 5). Scale bars are 200 μm ∗ and # indicate significant differences with respect to alginate and GO/alginate groups, respectively (p < 0.05).

Article Snippet: Human umbilical cord-derived MSCs and human umbilical vein endothelial cells (HUVEC) were obtained from PromoCell (Heidelberg, Germany).

Techniques: Staining

Journal: bioRxiv

Article Title: Capsaicin acts as a novel NRF2 agonist to suppress ethanol induced gastric mucosa oxidative damage by directly disrupting the KEAP1-NRF2 interaction

doi: 10.1101/2024.01.06.574490

Figure Lengend Snippet: (a) Microscopic examination of GES-1 cellular morphology following treatment regimens. Cells were pre-treated with Capsaicin (CAP) at concentrations of 2 or 8 μM for 2.5 hours, followed by incubation with 5% EtOH for 1.5 hours. Scale bar, 50 μm. (b) Detection of ROS-positive GES-1 cells using DCFH-DA staining. Scale bar, 200 μm. (c) Flow cytometric (FCM) analysis of ROS-positive GES-1 cells labeled with DCFH-DA. (d) Statistical analysis of ROS levels measured by FCM. The experiment was conducted in triplicate. (e) Assessment of SOD activity in GES-1 cells. (f) Quantification of MDA levels of GES-1 cells. (g) A network targets of CAP. Purple, blue, pink nodes represent proteins or genes in the predicted biological effect profile of CAP related to ROS, inflammation or immune, respectively. (h) Heatmap depicting differentially expressed genes (DEGs) enriched in antioxidant activity based on proteomic analysis. Each row represents the expression level of a single gene, while each column corresponds to an individual experimental sample. (i) Western blot analysis of NRF2 and HO-1 expression in GES-1 cells. (j) Western blot assessment of antioxidant protein expression in GES-1 cells. (k) Quantitative analysis of relative mRNA levels of antioxidant response elements (ARE)-related genes in GES-1 cells. Cells were treated with or without CAP and EtOH, and the mRNA levels of TXN, HMOX1, and NQO1 were measured. Each group consisted of three replicates. (l) Western blot analysis of NRF2 and HO-1 expression in human umbilical cord mesenchymal stem cells (HUC-MSCs). (m) Quantitative analysis of relative mRNA levels of ARE-related genes in HUC-MSCs. The mRNA levels of TXN, HMOX1, and NQO1 were measured in different treatment groups. Data were presented as mean ± SD. P-values were calculated using a T-test. Significance levels were indicated as follows: ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Human gastric mucosal epithelial cells (GES-1), HEK293T human embryonic kidney 293 cells (293T) and Human umbilical cord mesenchymal stem cells (UC-MSC) were obtained from the American Type Culture Collection (ATCC) and cultured at 37°C in 5% CO 2 .

Techniques: Incubation, Staining, Labeling, Activity Assay, Antioxidant Activity Assay, Expressing, Western Blot